A scientist explores the mysteries of the gut-brain connection |

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A friend’s gastric bypass surgery also fueled his curiosity. “A friend was struggling with obesity and, as a last resort, decided to have gastric bypass surgery. It worked. She lost a lot of weight, and it resolved her diabetes,” he recalls. “But most strikingly, her perception of taste changed. She used to be repulsed by the sight of runny egg yolks, but after the surgery, she craved them.” Such a change in taste has been well documented in some patients who’ve undergone bariatric surgery, but scientists aren’t sure how or why it happens, says Bohórquez. “It’s a new subject, but rewiring the gut appears to physically change how we perceive the taste of food in the brain.”

While scientists have known that nutrients are sensed in the gut by enteroendocrine cells, the exact way this happens was murky. They understood that when stimulated, enteroendocrine cells release hormones that either enter the bloodstream or activate nearby nerves to affect how we eat. “My focus has been to figure out how a sensory signal from a nutrient is transformed into an electrical signal that alters behavior,” Bohórquez says. He and his colleagues began taking a close look at enteroendocrine cells, using 3D electron microscopy. Imaging them in this way revealed a whole new structure that hadn’t been seen before. “It turns out enteroendocrine cells not only have microvilli, or tiny protrusions, exposed to the gut, but they also have a foot-like extension, which we called the neuropod,” says Bohórquez. “It became evident that enteroendocrine cells have similar physical attributes to neurons, so we wondered whether they might be wired to neurons, too.”

The secret to tracking synaptic connections: a special kind of rabies. The key to illuminating the process was inserting a tiny amount of modified fluorescent rabies virus into the colon of a mouse. “Rabies is a virus that infects neurons and spreads through synaptic connections, so when used in a modified form that only allows it to jump one neuron at a time, it’s useful for tracking neural circuits,” Bohórquez explains. Seven days after undergoing this procedure, the enteroendocrine cells of the mouse colon glowed green, offering evidence that the sensor cells were indeed behaving as neurons. Bohórquez then bred a mouse that would allow the tracking rabies to make a second jump. When he delivered the tracking rabies into the colon of this new mouse, the enteroendocrine cells and the nerves that they connected to lit up, demonstrating the existence of a physical synapse between the sensor cells and its nervous system — and a physical connection that hadn’t been seen before.

Charting the communication pathway between the gut and brain could someday lead us to new treatments for disorders and conditions. A number of diseases — autism, obesity, anorexia, irritable bowel syndrome, inflammatory bowel disease, PTSD and chronic stress — share a symptom known as altered visceral sensing, or a hyper- or hyposensitivity to gut stimuli. “For instance, clinical observations have suggested that some children with anorexia may be hyper-aware of the food they ingest from an early age,” says Bohórquez. “Under normal circumstances, this process happens without detailed spatial and temporal awareness, but those children can feel what’s going on in there, which triggers anxious feelings.” With this knowledge, scientists may better understand other disorders that have been thought to be solely psychological.

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